At the University of Portsmouth we have been able to establish an excellent suite of laboratories with tissue culture laboratories and a host of state-of-the-art equipment including those to enable various assays including histology, flow cytometry, cellular oxygen utilisation measurement, ECIS, TEER/Flocell/Cellix, Auto-MACs cell separation, cell viability, apoptosis & proliferation and RT-PCR, metabolic, gene and proteomic analysis which we have integrated into dedicated histology, cellular, molecular laboratories respectively. We have also established a sophisticated microscope imaging suite which includes atomic force, laser capture micro-dissection, confocal/live cell, total internal reflection (TIRF), Ariole quantitative image analysis and micro-fluidics/live cell microscopy as well as having full access to scanning and transmission electron microscopy and confocal microscopy. We have also developed a human biopsy-derived brain tumour cell culture repository holding well characterised (gene and protein levels), DNA fingerprinted, mycoplasma tested cells with an on line registry which we share with our collaborating laboratories without the UK.
We opened The Alison Phelan Memorial Laboratory in October 2012. The lab now houses molecular equipment, e.g. Western blotting (Bio-Rad MinoPROTEAN, Pierce PowerBlotter), PCR (Bio-rad MyCycler) etc, a 2100 Agilent Bioanalyser providing sizing, quantitation and quality control of DNA, RNA, proteins and cells (used in DNA fingerprinting and RNA integrity), a Licor Odyssey CLx Infrared… Continue reading Molecular Laboratory
With fume cupboards, Leica EG1150C FFPS processing/embedding stations, 3 rotary paraffin and 1 resin section Leica microtomes, a Leica CM3050C cryostat and tissue slicer.
Housing a Bruker Multimode and Nanoscope Atomic Force Microscope, a Leica Ariole brightfield, fluorescence and FISH slide microscope system with image analysis software, a Zeiss Total Internal Fluorescence Microscopy (Evanescence laser wave technology)/SIRF microscope, a Zeiss Inverted live-cell imaging microscope with environmental chamber for hypoxic and normoxic culture and Cellix microfluidics system, an AxioImager Z1 Fluorescence Inverted microscope… Continue reading Microscope Imaging Suite
Housing the Charlie’s Challenge room with epifluorescence/phase/brightfield Zeiss microscope, the Luke Frost room for establishing biopsies in culture and the Diana Ford room for generic tissue culture (each is fully equipped and with hypoxic and normoxic incubators). The Diana Ford room also houses Flocell, EVOHM/TEER, Electrical Cell Impedance Sensing and CellZscope physiological monitoring of TEER,… Continue reading Cellular Laboratory